Journal: Medicine

Article Title: Ferroptosis-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma: A multi-cohort retrospective study

doi: 10.1097/MD.0000000000047262

Figure Lengend Snippet: Clinical validation of FRGs mRNA expression. (A–L) The mRNA expression levels of key genes HIF1A (A), SRC (B), EZH2 (C), AR (D), SLC1A5 (E), KIF20A (F), SLC7A11 (G), CARS1 (H), MYCN (I), PRDX6 (J), GPX4 (K), and KLF2 (L) were further validated using 30 clinical HCC samples * P < .05; ** P < .01; *** P < .001; **** P < .0001. AR = androgen receptor; ns: no statistical significance.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies: SLC1A5 (1:500, 20350-1-AP, Proteintech), KIF20A (1:200, 15911-1-AP, Proteintech), SLC7A11 (1:200, 26864-1-AP, Proteintech), CARS1 (1:500, 15296-1-AP, Proteintech), MYCN (1:250, 10159-2-AP, Proteintech), PRDX6 (1:100, 13585-1-AP, Proteintech), GPX4 (1:2000, 67763-1-Ig, Proteintech), and KLF2 (1:200, bs-2772R, Bioss), and then with the secondary antibody for 1 hour at 24 °C.

Techniques: Biomarker Discovery, Expressing

Journal: Medicine

Article Title: Ferroptosis-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma: A multi-cohort retrospective study

doi: 10.1097/MD.0000000000047262

Figure Lengend Snippet: Clinical validations of FRGs protein expression. (A–H) Representative images of immunohistochemical staining for SLC1A5 (A), KIF20A (B), SLC7A11 (C), CARS1 (D), MYCN (E), PRDX6 (F), GPX4 (G), and KLF2 (H) in tumor and para-tumor tissues. Scale bars: 50 μm (main), 25 μm (insets). (I–P) Mean optical density (MOD) of FRGs proteins in tumor and para-tumor tissues, including SLC1A5 (I), KIF20A (J), SLC7A11 (K), CARS1 (L), MYCN (M), PRDX6 (N), GPX4 (O), and KLF2 (P). * P < .05; ** P < .01; **** P < .0001. ns = no statistical significance.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies: SLC1A5 (1:500, 20350-1-AP, Proteintech), KIF20A (1:200, 15911-1-AP, Proteintech), SLC7A11 (1:200, 26864-1-AP, Proteintech), CARS1 (1:500, 15296-1-AP, Proteintech), MYCN (1:250, 10159-2-AP, Proteintech), PRDX6 (1:100, 13585-1-AP, Proteintech), GPX4 (1:2000, 67763-1-Ig, Proteintech), and KLF2 (1:200, bs-2772R, Bioss), and then with the secondary antibody for 1 hour at 24 °C.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: (a) White arrows indicate the localization of IBV N protein in AQP2-expressing collecting duct cells. Green fluorescence shows positive staining for IBV N and red fluorescence shows staining for AQP2. (b) White arrows indicate the localization of IBV N protein in CALB1-expressing distal tubule cells. Red fluorescence shows positive staining for IBV N and green fluorescence shows staining for CALB1. (c) Changes in cell communication numbers: The top network diagram shows cell clusters as nodes, with line thickness indicating changes in communication numbers. The lower heatmap details these changes, with rows representing signal-sending cells and columns indicating signal-receiving cells. The color scale reflects the inter-group differences in signal communication frequency between different cell types (number of communications in the infected group—number in control group). The bar plots at the top and right side represent the overall differences in the number of signals sent/received by specific cell clusters. (d) Changes in cell communication strength: Similar to (c), with the top network diagram displaying changes in communication strength (communication strength in the infected group—strength in the control group). In the lower heatmap, the color scale reflects the inter-group differences in signal communication strength between different cell types. The bar plots at the top and right side represent the overall differences in the strength of signals sent/received by specific cell clusters (infected group—control group). (e) Inter-group differences in the communication strength of specific signaling pathways (receptor-ligand pairs) across cell clusters. Rows represent signal pathways and columns correspond to cell clusters, with heatmap colors depicting the strength variation of signals (infect group vs. control group). Upper left triangles for signal sent and lower right triangles for signal received. The bar plot on the right side shows the overall difference in communication strength of these signaling pathways between the IBV-infected group and the control group.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Expressing, Fluorescence, Staining, Infection, Control, Protein-Protein interactions

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: Antbodies and reagents used in present study.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Blocking Assay, Immunofluorescence